Biological Mass Spectrometry (BMS)
The BMS sub-unit of the ABRF specialises in the analysis of protein and peptides. The BMS sub-unit currently has three instruments (plus additional support equipment):
- Ion Trap Mass spectrometer with ETD
- Quadrupole –Time of Flight (Q-ToF)
- Matrix Assisted Laser Desorption Ionisation -ToF/ToF (MALDI TOF/TOF)
The ABRF is equipped with a Thermo-Finnegan LTQ XL with ETD module allowing MSn analysis of peptides and multiple options for peptide dissociation: including CID, ETD and PQD.
For more information on applications and capabilities of the Ion Trap click here.
The ABRF’s Q-ToF is a Bruker Daltonics micrOTOF-Q II, an advanced quadrupole time-of-flight mass spectrometer providing high resolution (17,500 FWHM) and mass accuracy (<5 ppm) efficient TOF scan rates of 20kHz allowing 4 MS + 16 MS/MS spectra per second, and an affective mass range of 50 to 20,000 m/z suitable for Proteomics, Metabolomics, Small Molecule Identification and Quantitation (label and label free).
For more information on applications and capabilities of the Q-ToF click here.
The ABRF also features a Bruker Daltonics UltrafleXtreme MALDI TOF/TOF a next generation MALDI TOF/TOF providing ultra high resolution (exceeding 40,000 FWHM in MS mode), 1 ppm mass accuracy in both MS and MS/MS mode and ultra fast acquisition (1 kHz laser repetition rate) making the UltrafleXtreme suitable for Proteomics, Quantitation, Top-Down Sequencing and MALDI Imaging.
For more information on applications and capabilities of the MALDI ToF/ToF click here.