2021 |
Damanhuri HA, Dunkley PR, Goodchild AK, 'The effects of acute glucoprivation on adrenomedullary function in SHR and WKY rats', Sains Malaysiana, 50 481-492 (2021) [C1]
We have shown previously, acute intraperitoneal administration of 2-deoxy-d-glucose (2DG) into Sprague-Dawley rats led to activation of the adrenal medulla chromaffin cells, indic... [more]
We have shown previously, acute intraperitoneal administration of 2-deoxy-d-glucose (2DG) into Sprague-Dawley rats led to activation of the adrenal medulla chromaffin cells, indicated with increased protein kinase activity and increased tyrosine hydroxylase (TH) phosphorylation, as well as increased plasma adrenaline and glucose levels. Here we have used spontaneous hypertensive (SHR) and Wistar Kyoto (WKY) rats to investigate whether hypertension alters basal adrenal chromaffin cell function, or the response of these cells to acute 2DG treatment. At basal level, we found no differences in adrenal medulla TH protein, TH phosphorylation, TH activity or catecholamine levels between SHR and WKY despite a significant difference in the level of systolic blood pressure; nor were there differences in plasma catecholamine levels or blood glucose (BG). Furthermore, the vehicle animals evoked no significant changes in any parameter measured in SHR, but evoked significant increases in pSer19TH, plasma adrenaline and BG in WKY. Single episode of glucoprivation evoked increases in PKA and CDK/MAPK, pSer40TH, pSer31TH, TH activity, and plasma adrenaline and BG in SHR, and in addition evoked increases in PKC, CAMKII, and pSer19TH in WKY. These findings are significant which indicates hypertension does not impact catecholamine function in the adrenal gland. It also appears that hypertension does not alter the adrenal response to glucoprivation. The findings are also significant as WKY showed greater adrenal activation of protein kinases and TH phosphorylation in response to saline and 2DG when compared to SHR and possible reasons for these findings are further discussed.
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Nova |
2021 |
Ong LK, Briggs GD, Guan L, Dunkley PR, Dickson PW, 'Peripheral inflammation induces long-term changes in tyrosine hydroxylase activation in the substantia nigra', NEUROCHEMISTRY INTERNATIONAL, 146 (2021) [C1]
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Nova |
2019 |
Shehadeh J, Double KL, Murphy KE, Bobrovskaya L, Reyes S, Dunkley PR, et al., 'Expression of tyrosine hydroxylase isoforms and phosphorylation at serine 40 in the human nigrostriatal system in Parkinson's disease', Neurobiology of Disease, 130 (2019) [C1]
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Nova |
2019 |
Dunkley PR, Dickson PW, 'Tyrosine hydroxylase phosphorylation in vivo', Journal of Neurochemistry, 149 706-728 (2019) [C1]
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, noradrenaline and adrenaline. One of the major mechanisms for controlling th... [more]
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, noradrenaline and adrenaline. One of the major mechanisms for controlling the activity of TH is protein phosphorylation. TH is phosphorylated at serine residues 8, 19, 31 and 40. There have been a number of previous reviews focused on TH phosphorylation in¿vitro and in¿situ. This review on TH phosphorylation in¿vivo has three main sections focusing on: (1) the methods used to investigate TH phosphorylation in¿vivo, including the animals used, the sacrifice procedures, the tissue preparation, the measurement of TH protein levels and TH phosphorylation and the measurement of TH activation. (2) The regulation of TH phosphorylation and its consequences in¿vivo, including the kinases and phosphatases acting on TH, the stoichiometry of TH phosphorylation, the proteins that bind TH and TH subcellular location. (3) The acute and prolonged TH phosphorylation changes in specific catecholaminergic tissues, including the adrenal medulla, the nigrostriatal pathway and the mesolimbic pathway. (Figure presented.).
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Nova |
2019 |
Kunzler A, Garcia Sobrinho P, Smith T, Gelain DP, Moreira JCF, Dunkley PR, Dickson PW, 'Subcellular distribution of human tyrosine hydroxylase isoforms 1 and 4 in SH-SY5Y cells', Journal of Cellular Biochemistry, 120 19730-19737 (2019) [C1]
Tyrosine hydroxylase (TH) is the key enzyme that controls the rate of synthesis of the catecholamines. SH-SY5Y cells with stable transfections of either human tyrosine hydroxylase... [more]
Tyrosine hydroxylase (TH) is the key enzyme that controls the rate of synthesis of the catecholamines. SH-SY5Y cells with stable transfections of either human tyrosine hydroxylase isoform 1 (hTH1) or human tyrosine hydroxylase isoform 4 (hTH4) were used to determined the subcellular distribution of TH protein and phosphorylated TH, under basal conditions and after muscarine stimulation. Muscarine was previously shown to increase the phosphorylation of only serine 19 and serine 40 in hTH1 cells. Under basal conditions, the hTH1 and hTH4 proteins, their serine 19 phosphorylated forms and hTH1 phosphorylated at serine 40 were all similarly distributed; with ~80% in the cytosolic fraction, ~20% in the membrane fraction, and less than 1%, or not detectable, in the nuclear fraction. However, hTH4 phosphorylated at serine 71 had a significantly different distribution with ~65% cytosolic and ~35% membrane associated. Muscarine stimulation led to hTH1 being redistributed from the cytosol and nuclear fractions to the membrane fraction and hTH4 being redistributed from the cytosol to the nuclear fraction. These muscarine stimulated redistributions were not due to TH phosphorylation at serine 19, serine 40, or serine 71 and were most likely due to TH binding to proteins whose phosphorylation was increased by muscarine. This is the first study to show a difference in subcellular distribution between two human TH isoforms under basal and stimulated conditions.
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Nova |
2017 |
Ong LK, Fuller EA, Sominsky L, Hodgson DM, Dunkley PR, Dickson PW, 'Early life peripheral lipopolysaccharide challenge reprograms catecholaminergic neurons', SCIENTIFIC REPORTS, 7 (2017) [C1]
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Nova |
2017 |
Ong LK, Page S, Briggs GD, Guan L, Dun MD, Verrills NM, et al., 'Peripheral Lipopolysaccharide Challenge Induces Long-Term Changes in Tyrosine Hydroxylase Regulation in the Adrenal Medulla', Journal of Cellular Biochemistry, 118 2096-2107 (2017) [C1]
Immune activation can alter the activity of adrenal chromaffin cells. The effect of immune activation by lipopolysaccharide (LPS) on the regulation of tyrosine hydroxylase (TH) in... [more]
Immune activation can alter the activity of adrenal chromaffin cells. The effect of immune activation by lipopolysaccharide (LPS) on the regulation of tyrosine hydroxylase (TH) in the adrenal medulla in vivo was determined between 1 day and 6 months after LPS injection. The plasma levels of eleven cytokines were reduced 1 day after LPS injection, whereas the level for interleukin-10 was increased. The levels of all cytokines remained at control levels until 6 months when the levels of interleukin-6 and -4 were increased. One day after LPS injection, there was a decrease in TH-specific activity that may be due to decreased phosphorylation of serine 31 and 40. This decreased phosphorylation of serine 31 and 40 may be due to an increased activation of the protein phosphatase PP2A. One week after LPS injection, there was increased TH protein and increased phosphorylation of serine 40 that this was not accompanied by an increase in TH-specific activity. All TH parameters measured returned to basal levels between 1 month and 3 months. Six months after injection there was an increase in TH protein. This was associated with increased levels of the extracellular regulated kinase isoforms 1 and 2. This work shows that a single inflammatory event has the capacity to generate both short-term and long-term changes in TH regulation in the adrenal medulla of the adult animal. J. Cell. Biochem. 118: 2096¿2107, 2017. © 2016 Wiley Periodicals, Inc.
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Nova |
2017 |
Gasparotto J, Ribeiro CT, Bortolin RC, Somensi N, Fernandes HS, Teixeira AA, et al., 'Anti-RAGE antibody selectively blocks acute systemic inflammatory responses to LPS in serum, liver, CSF and striatum', Brain, Behavior, and Immunity, 62 124-136 (2017) [C1]
Systemic inflammation induces transient or permanent dysfunction in the brain by exposing it to soluble inflammatory mediators. The receptor for advanced glycation endproducts (RA... [more]
Systemic inflammation induces transient or permanent dysfunction in the brain by exposing it to soluble inflammatory mediators. The receptor for advanced glycation endproducts (RAGE) binds to distinct ligands mediating and increasing inflammatory processes. In this study we used an LPS-induced systemic inflammation model in rats to investigate the effect of blocking RAGE in serum, liver, cerebrospinal fluid (CSF) and brain (striatum, prefrontal cortex, ventral tegmental area and substantia nigra). Intraperitoneal injection of RAGE antibody (50¿µg/kg) was followed after 1¿h by a single LPS (5¿mg/kg) intraperitoneal injection. Twenty-four hours later, tissues were isolated for analysis. RAGE antibody reduced LPS-induced inflammatory effects in both serum and liver; the levels of proinflammatory cytokines (TNF-a, IL-1ß) were decreased and the phosphorylation/activation of RAGE downstream targets (ERK1/2, I¿B and p65) in liver were significantly attenuated. RAGE antibody prevented LPS-induced effects on TNF-a and IL-1ß in CSF. In striatum, RAGE antibody inhibited increases in IL-1ß, Iba-1, GFAP, phospho-ERK1/2 and phospho-tau (ser202), as well as the decrease in synaptophysin levels. These effects were caused by systemic RAGE inhibition, as RAGE antibody did not cross the blood-brain barrier. RAGE antibody also prevented striatal lipoperoxidation and activation of mitochondrial complex II. In conclusion, blockade of RAGE is able to inhibit inflammatory responses induced by LPS in serum, liver, CSF and brain.
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Nova |
2017 |
Kunzler A, Zeidán-Chuliá F, Gasparotto J, Girardi CS, Klafke K, Petiz LL, et al., 'Changes in Cell Cycle and Up-Regulation of Neuronal Markers During SH-SY5Y Neurodifferentiation by Retinoic Acid are Mediated by Reactive Species Production and Oxidative Stress', Molecular Neurobiology, 54 6903-6916 (2017) [C1]
Human neuroblastoma SH-SY5Y cells have been used as an in vitro model for neurodegenerative disorders such as Parkinson¿s disease and can be induced to a mature neuronal phenotype... [more]
Human neuroblastoma SH-SY5Y cells have been used as an in vitro model for neurodegenerative disorders such as Parkinson¿s disease and can be induced to a mature neuronal phenotype through retinoic acid (RA) differentiation. However, mechanisms of RA-induced differentiation remain unclear. Here, we investigate the role of reactive species (RS) on SH-SY5Y neuroblastoma cells under RA differentiation, using the antioxidant Trolox® as co-treatment. We found that RA treatment for 7¿days reduced the cell number and proliferative capacity and induced the expression of adult catecholaminergic/neuronal markers such as tyrosine hydroxylase (TH), ß-III tubulin, and enolase-2. Evaluation of intracellular RS production by DCFH oxidation assay and quantification of cell non-enzymatic antioxidant activity by TRAP demonstrated that RA increases RS production. Furthermore, mitochondrial NADH oxidation showed to be inhibited under differentiation with RA. Cells subjected to co-treatment with antioxidant Trolox® demonstrated a remaining proliferative capacity and a decrease in the pro-oxidant state and RS production. Besides, antioxidant treatment restores the mitochondrial NADH oxidation. Importantly, Trolox® co-treatment inhibited the appearance of morphological characteristics such as neurite extension and branching, and decreased the expression of TH, ß-III tubulin, and enolase-2 after a seven-day differentiation with RA, indicating that RS production is a necessary step in this process. Trolox® also inhibited the phosphorylation of Akt and ERK1/2, which are involved in differentiation and survival, respectively, of these cells. Altogether, these data indicate the presence of a redox-dependent mechanism in SH-SY5Y RA-differentiation process and can be a useful insight to improve understanding of neuronal differentiation signaling.
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Nova |
2016 |
Peres TV, Ong LK, Costa AP, Eyng H, Venske DKR, Colle D, et al., 'Tyrosine hydroxylase regulation in adult rat striatum following short-term neonatal exposure to manganese', Metallomics, 8 597-604 (2016) [C1]
Manganese (Mn) is an essential trace element required for a range of physiological processes, but Mn can also be neurotoxic especially during development. Excess levels of Mn accu... [more]
Manganese (Mn) is an essential trace element required for a range of physiological processes, but Mn can also be neurotoxic especially during development. Excess levels of Mn accumulate preferentially in the striatum and can induce a syndrome called manganism, characterized by an initial stage of psychiatric disorder followed by motor impairment. In the present study, we investigated the effects of Mn exposure on the developing dopaminergic system, specifically tyrosine hydroxylase (TH) protein and phosphorylation levels in the striatum of rats. Neonatal rats were exposed to Mn intraperitoneally (ip) from post-natal day 8 up to day 12 (PND8-12). Striatal tissue was analysed on PND14 or PND70, to detect either short-term or long-term effects induced by Mn exposure. There was a dose dependent increase in TH protein levels in the striatum at PND14, reaching significance at 20 mg kg-1 Mn, and this correlated with an increase in TH phosphorylation at serines 40, 31 and 19. However, in the striatum at PND70, a time by which Mn levels were no longer elevated, there was a dose dependent decrease in TH protein levels, reaching significance at 20 mg kg-1 Mn, and this correlated with TH phosphorylation at Ser40 and Ser19. There was however a significant increase in phosphorylation of TH at serine 31 at 20 mg kg-1 Mn, which did not correlate with TH protein levels. Taken together our findings suggest that neonatal Mn exposure can have both short-term and long-term effects on the regulation of TH in the striatal dopaminergic system.
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Nova |
2016 |
de Miranda Ramos V, Zanotto-Filho A, de Bittencourt Pasquali MA, Klafke K, Gasparotto J, Dunkley P, et al., 'NRF2 Mediates Neuroblastoma Proliferation and Resistance to Retinoic Acid Cytotoxicity in a Model of In Vitro Neuronal Differentiation', Molecular Neurobiology, 53 6124-6135 (2016) [C1]
Retinoic acid (RA) morphogenetic properties have been used in different kinds of therapies, from neurodegenerative disorders to some types of cancer such as promyelocytic leukemia... [more]
Retinoic acid (RA) morphogenetic properties have been used in different kinds of therapies, from neurodegenerative disorders to some types of cancer such as promyelocytic leukemia and neuroblastoma. However, most of the pathways responsible for RA effects remain unknown. To investigate such pathways, we used a RA-induced differentiation model in the human neuroblastoma cells, SH-SY5Y. Our data showed that n-acetyl-cysteine (NAC) reduced cells¿ proliferation rate and increased cells¿ sensitivity to RA toxicity. Simultaneously, NAC pre-incubation attenuated nuclear factor erythroid 2-like factor 2 (NRF2) activation by RA. None of these effects were obtained with Trolox® as antioxidant, suggesting a cysteine signalization by RA. NRF2 knockdown increased cell sensibility to RA after 96¿h of treatment and diminished neuroblastoma proliferation rate. Conversely, NRF2 overexpression limited RA anti-proliferative effects and increased cell proliferation. In addition, a rapid and non-genomic activation of the ERK 1/2 and PI3K/AKT pathways revealed to be equally required to promote NRF2 activation and necessary for RA-induced differentiation. Together, we provide data correlating NRF2 activity with neuroblastoma proliferation and resistance to RA treatments; thus, this pathway could be a potential target to optimize neuroblastoma chemotherapeutic response as well as in vitro neuronal differentiation protocols.
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Nova |
2014 |
Ong LK, Guan L, Damanhuri H, Goodchild AK, Bobrovskaya L, Dickson PW, Dunkley PR, 'Neurobiological consequences of acute footshock stress: effects on tyrosine hydroxylase phosphorylation and activation in the rat brain and adrenal medulla', JOURNAL OF NEUROCHEMISTRY, 128 547-560 (2014) [C1]
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Nova |
2014 |
Stutz B, Lima da Conceicao FS, Santos LE, Cadilhe DV, Fleming RL, Acquarone M, et al., 'Murine dopaminergic Muller cells restore motor function in a model of Parkinson's disease', JOURNAL OF NEUROCHEMISTRY, 128 829-840 (2014) [C1]
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Nova |
2013 |
Briggs G, Bulley J, Dunkley PR, Dickson PW, 'Structural Basis for Regulation of Tyrosine Hydroxlyase by the Catecholamines 8-9 (2013) [E3]
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2013 |
Bobrovskaya L, Maniam J, Ong LK, Dunkley PR, Morris MJ, 'Early Life Stress and Post-Weaning High Fat Diet Alter Tyrosine Hydroxylase Regulation and AT1 Receptor Expression in the Adrenal Gland in a Sex Dependent Manner', NEUROCHEMICAL RESEARCH, 38 826-833 (2013) [C1]
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Nova |
2013 |
Sominsky L, Fuller EA, Bondarenko E, Ong LK, Averell L, Nalivaiko E, et al., 'Functional Programming of the Autonomic Nervous System by Early Life Immune Exposure: Implications for Anxiety', PLOS ONE, 8 (2013) [C1]
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Nova |
2012 |
Dunkley P, 'Richard Burnard Rodnight, 1921-2012.', Journal of neurochemistry, 123 199-201 (2012)
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2012 |
Dunkley PR, 'Richard Burnard Rodnight, 1921-2012 [Obituary]', Journal of Neurochemistry, 123 199-201 (2012) [C3]
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2012 |
Ong LK, Sominsky L, Dickson PW, Hodgson DM, Dunkley PR, 'The sustained phase of Tyrosine hydroxylase activation in vivo', Neurochemical Research, 37 1938-1943 (2012) [C1]
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Nova |
2012 |
Barreto RDA, Walker FR, Dunkley PR, Day TA, Smith DW, 'Fluoxetine prevents development of an early stress-related molecular signature in the rat infralimbic medial prefrontal cortex. Implications for depression?', BMC Neuroscience, 13 1-12 (2012) [C1]
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Nova |
2012 |
Dayas CV, Smith DW, Dunkley PR, 'An emerging role for the mammalian Target of Rapamycin (mTOR) in 'pathological' protein translation: Relevance to cocaine addiction', Frontiers in Pharmacology, 3 1-12 (2012) [C1]
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Nova |
2012 |
Damanhuri HA, Burke PGR, Ong LK, Bobrovskaya L, Dickson PW, Dunkley PR, Goodchild AK, 'Tyrosine hydroxylase phosphorylation in catecholaminergic brain regions: A marker of activation following acute hypotension and glucoprivation', Plos One, 7 1-19 (2012) [C1]
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Nova |
2011 |
Ong LK, Guan L, Stutz B, Dickson PW, Dunkley PR, Bobrovskaya L, 'The effects of footshock and immobilization stress on tyrosine hydroxylase phosphorylation in the rat locus coeruleus and adrenal gland', Neuroscience, 192 20-27 (2011) [C1]
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Nova |
2011 |
Ong LK, Bobrovskaya L, Walker FR, Day TA, Dickson PW, Dunkley PR, 'The effect of social defeat on tyrosine hydroxylase phosphorylation in the rat brain and adrenal gland', Neurochemical Research, 36 27-33 (2011) [C1]
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Nova |
2010 |
Bobrovskaya L, Damanhuri HA, Ong LK, Schneider JJ, Dickson PW, Dunkley PR, Goodchild AK, 'Signal transduction pathways and tyrosine hydroxylase regulation in the adrenal medulla following glucoprivation: An in vivo analysis', Neurochemistry International, 57 162-167 (2010) [C1]
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Nova |
2010 |
Double KL, Halliday GM, Dunkley PR, Dickson PW, Gerlach M, Riederer P, 'Pigmentation in the human brain and risk of Parkinson's Disease', Annals of Neurology, 67 553-554 (2010) [C3]
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2010 |
Posser T, Dunkley PR, Dickson PW, Franco JL, 'Human neuroblastoma cells transfected with tyrosine hydroxylase gain increased resistance to methylmercury-induced cell death', Toxicology in Vitro, 24 1498-1503 (2010) [C1]
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Nova |
2010 |
Franco JL, Posser T, Gordon SL, Bobrovskaya L, Schneider JJ, Farina M, et al., 'Expression of tyrosine hydroxylase increases the resistance of human neuroblastoma cells to oxidative insults', Toxicological Sciences, 113 150-157 (2010) [C1]
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Nova |
2009 |
Posser T, Franco JL, Bobrovskaya L, Leal RB, Dickson PW, Dunkley PR, 'Manganese induces sustained Ser40 phosphorylation and activation of tyrosine hydroxylase in PC12 cells', Journal of Neurochemistry, 110 848-856 (2009) [C1]
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Nova |
2009 |
Gordon SL, Bobrovskaya L, Dunkley PR, Dickson PW, 'Differential regulation of human tyrosine hydroxylase isoforms 1 and 2 in situ: Isoform 2 is not phosphorylated at Ser35', Biochimica et Biophysica Acta - Molecular Cell Research, 1793 1860-1867 (2009) [C1]
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Nova |
2009 |
Gordon SL, Webb JK, Shehadeh J, Dunkley PR, Dickson PW, 'The low affinity dopamine binding site on tyrosine hydroxylase: The role of the N-Terminus and in situ regulation of enzyme activity', Neurochemical Research, 34 1830-1837 (2009) [C1]
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Nova |
2009 |
Franco JL, Posser T, Dunkley PR, Dickson PW, Mattos JJ, Martins R, et al., 'Methylmercury neurotoxicity is associated with inhibition of the antioxidant enzyme glutathione peroxidase', Free Radical Biology and Medicine, 47 449-457 (2009) [C1]
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Nova |
2008 |
Dunkley PR, Jarvie P, Robinson PJ, 'A rapid Percoll gradient procedure for preparation of synaptosomes', Nature Protocols, 3 1718-1728 (2008) [C1]
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Nova |
2008 |
Gordon SL, Quinsey NS, Dunkley PR, Dickson PW, 'Tyrosine hydroxylase activity is regulated by two distinct dopamine-binding sites', Journal of Neurochemistry, 106 1614-1623 (2008) [C1]
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Nova |
2007 |
Bobrovskaya L, Gelain DP, Gilligan C, Dickson PW, Dunkley PR, 'PACAP stimulates the sustained phosphorylation of tyrosine hydroxylase at serine 40', Cellular Signalling, 19 1141-1149 (2007) [C1]
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2007 |
Bobrovskaya L, Gilligan C, Bolster EK, Flaherty JJ, Dickson PW, Dunkley PR, 'Sustained phosphorylation of tyrosine hydroxylase at serine 40: a novel mechanism for maintenance of catecholamine synthesis', Journal of Neurochemistry, 100 479-489 (2007) [C1]
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2007 |
Gelain DP, Moreira JCF, Bevilaqua LRM, Dickson PW, Dunkley PR, 'Retinol activates tyrosine hydroxylase acutely by increasing the phosphorylation of serine40 and then serine31 in bovine adrenal chromaffin cells', Journal of Neurochemistry, 103 2369-2379 (2007) [C1]
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2007 |
Leal RB, Posser T, Rigon AP, Oliveira CS, Goncalves CA, Gelain DP, Dunkley PR, 'Cadmium stimulates MAPKs and Hsp27 phosphorylation in bovine adrenal chromaffin cells', Toxicology, 234 34-43 (2007) [C1]
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2006 |
Lehmann IT, Bobrovskaya L, Gordon SL, Dunkley PR, Dickson PW, 'Differential regulation of the human tyrosine hydroxylase isoforms via hierarchical phosphorylation', Journal of Biological Chemistry, 281 17644-17651 (2006) [C1]
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Nova |
2005 |
Cechin SR, Dunkley PR, Rodnight RB, 'Signal transduction mechanisms involved in the proliferation of C6 glioma cells induced by lysophosphatidic acid', Neurochemical Research, 30 603-611 (2005) [C1]
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Nova |
2005 |
Graham ME, Dickson PW, Dunkley PR, Von Nagy-Felsobuki EI, 'Characterisation of tryptic peptides of phosphorylated tyrosine hydroxylase by high-pressure liquid chromatography electrospray ionisation mass spectrometry', Journal of Electron Spectroscopy and Related Phenomena, 142 271-276 (2005) [C1]
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Nova |
2004 |
Cordova FM, Rodrigues ALS, Giacomelli MBO, Oliveira CS, Posser T, Dunkley PR, Leal RB, 'Lead stimulates ERK1/2 and p38MAPK phosphorylation in the hippocampus of immature rats', Brain Research, 998 65-72 (2004) [C1]
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2004 |
Ma FY, Grattan DR, Bobrovskaya L, Dunkley PR, Bunn SJ, 'Angiotensin II regulates tyrosine hydroxylase activity and mRNA expression in rat mediobasal hypothalamic cultures: the role of specific protein kinases', Journal of Neurochemistry, 90 431-441 (2004) [C1]
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2004 |
Dunkley PR, Bobrovskaya L, Graham ME, Von Nagy-Felsobuki EI, Dickson PW, 'Tyrosine hydroxylase phosphorylation: regulation and consequences', Journal of Neurochemistry, 91 1025-1043 (2004) [C1]
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Nova |
2004 |
Roberts-Thomson EL, Herd LM, Saunders H, Dunkley PR, Bunn SJ, 'The tryrosine phosphorylation and cytoskeletal translocation of phospholipase Cy1 in bovine adrenal medullary chromaffin cells', Neurochemical Research, 29 1847-1855 (2004) [C1]
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2004 |
Frizzo JK, Tramontina F, Bortoli E, Gottfried C, Leal RB, Lengyel I, et al., 'S100B-Mediated Inhibition of the Phosphorylation of GFAP Is Prevented by TRTK-12', Neurochemical Research, 29 735-740 (2004) [C1]
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2004 |
Roberts-Thomson EL, Herd LM, Saunders HI, Dunkley PR, Bunn SJ, 'The tyrosine [correction of tryrosine] phosphorylation and cytoskeletal translocation of phospholipase C gamma 1 in bovine adrenal medullary chromaffin cells.', Neurochemical research, 29 1847-1855 (2004)
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2004 |
Leal RB, Frizzo JK, Tramontina F, Fieuw-Makaroff S, Bobrovskaya L, Dunkley PR, Goncalves CA, 'S1009B protein stimulates calcineurin activity', NEUROREPORT, 15 317-320 (2004)
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2004 |
Leal RB, Frizzo JK, Tramontina F, Fieuw-Makaroff S, Bobrovskaya L, Dunkley PR, Goncalves C-A, 'S100B protein stimulates calcineurin activity', NeuroReport, 15 317-320 (2004) [C1]
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2003 |
Cammarota MP, Bevilaqua L, Rostas JA, Dunkley PR, 'Histamine activates tyrosine hydroxylase in bovine adrenal chromaffin cells through a pathway that involves ERK1/2 but not p38 or JNK', Journal of Neurochemistry, 84 453-458 (2003) [C1]
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2003 |
Bevilaqua L, Cammarota MP, Dickson PW, Sim AT, Dunkley PR, 'Role of protein phosphatase 2C from bovine adrenal chromaffin cells in the dephosphorylation of phospho-serine 40 tyrosine hydroxylase', Journal of Neurochemistry, 85 1368-1373 (2003) [C1]
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2003 |
Powis DA, Zerbes M, Herd LM, Dunkley PR, 'Angiotensin II Causes Calcium Entry into Bovine Adrenal Chromaffin Cells via Pathway(s) Activated by Depletion of Intracellular Calcium Stores', Neurochemical Research, 28 1299-1306 (2003) [C1]
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2002 |
Leal R, Cordova F, Herd LM, Bobrovskaya L, Dunkley PR, 'Lead-Stimulated p38 MARK-Dependent Hsp27 Phosphorylation', Toxicology and Applied Pharmacology, 178 44-51 (2002) [C1]
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Nova |
2002 |
Leal R, Sim AT, Goncalves C, Dunkley PR, 'Tyrosine Hydroxylase Dephosphorylation by Protein Phosphatase 2A in Bovine Adrenal Chromaffin Cells', Neurochemical Research, 27, No 3 207-213 (2002) [C1]
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2002 |
Warden RA, Noltorp RA, Bell CJ, O'Loughlin EV, Dunkley PR, Garg ML, 'Vitamin A Deficiency Changes Jejunal Mucosal Fatty Acid Profile in Rats', Journal of Clinical Biochemistry and Nutrition, 31 19-26 (2002) [C1]
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2002 |
Graham ME, Bevilaqua LRM, Phillip, Dickson W, Dunkley PR, Von Nagy-Felsobuki EI, 'Kinetic monitoring of protein phosphorylation using quantitative electrospray lonization mass spectrometry', Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, 301-302 (2002)
A kinetic model of protein phosphorylation using quantitative electrospray ionization mass spectrometry (ESI-MS) was discussed. Rate constants were determined in the absence of do... [more]
A kinetic model of protein phosphorylation using quantitative electrospray ionization mass spectrometry (ESI-MS) was discussed. Rate constants were determined in the absence of dopamine only. The analysis showed that the rate of serine 40 phosphorylation was two to three times higher when serine 19 was already phosphorylated. The application of quantitative ESI-MS to enzyme kinetics to provide information on protein structure/function was also demonstrated.
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2001 |
Bevilaqua L, Graham ME, Dunkley PR, Von Nagy-Felsobuki EI, Dickson PW, 'Phosphorylation of Ser19 Alters the Conformation of Tyrosine Hydroxylase to Increase the Rate of Phosphorylation of Ser40*', The Journal of Biological Chemistry, 276 No. 44 40411-40416 (2001) [C1]
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Nova |
2001 |
Leal RB, Cordova FM, Lynn H, Dunkley PR, 'Lead and cadmium stimulate HSP27 phosphorylation in bovine adrenal chromaffin cells', JOURNAL OF NEUROCHEMISTRY, 78 194-194 (2001) |
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2001 |
Bobrovskaya L, Odell AF, Leal RB, Dunkley PR, 'Tyrosine hydroxylase phosphorylation in bovine adrenal chromaffin cells: the role of MAPKs after angiotensin II stimulation', Journal of Neurochemistry, 78 490-498 (2001) [C1]
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2001 |
Cammarota MP, Bevilaqua L, Dunkley PR, Rostas JA, 'Angiotensin II Promotes the Phosphorylation of Cyclic AMP-Responsive Element Binding Protein (CREB) at Ser133 Through an ERK1/2-Dependent Mechanism', Journal of Neurochemistry, 79 1122-1128 (2001) [C1]
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2001 |
Bevilaqua L, Graham ME, Von Nagy-Felsobuki EI, Dunkley PR, Dickson PW, 'Effect of phosphorylation on tyrosine hydroxylase shape', Journal of Neurochemistry, 78 Supt. 1 143 (2001) [C3]
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2001 |
Cammarota MP, Bevilaqua L, Dunkley PR, Rostas JA, 'Angiotensin II Promotes a Rapid and Reversible Increase in SRC-Tyrosine Kinase Activity in Bovine Adrenal Chromaffin Cells: Effect on MAPK Pathways', Proceedings of the Australian Neuroscience Society, 12 197 (2001) [C3]
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2001 |
Bobrovskaya L, Odell AF, Cheah TB, Dunkley PR, 'The Involvement of MAPKs in Angiotensin II-Stimulated Tyrosine Hydroxylase Phosphorylation in Bovine Adrenal Chromaffin Cells', Proceedings of the Australian Neuroscience Society, 12 85 (2001) [C3] |
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2001 |
Graham ME, Bevilaqua L, Dunkley PR, Von Nagy-Felsobuki EI, Dickson PW, 'The Kinetics of CAMKII Phosphorylation of Dopamine Bound-Tyrosine Hydroxylase Determined by Electrospray Mass Spectrometry', ComBio 2001, 0 (2001) [C3]
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2001 |
Cammarota MP, Bevilaqua L, Dunkley PR, Rostas JA, 'Angiotensin II Promotes a Src and ERK 1/2 Dependent Increase in SER133-CREB Phosphorylation', Journal of Neurochemistry, 78 Supl. 1 143 (2001) [C3]
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2000 |
Karl J, Gottfried C, Tramontina F, Dunkley PR, Rodnight R, Goncalves CA, 'GFAP phosphorylation studied in digitonin-permeabilized astrocytes: standardization of conditions', Brain Research, 853 32-40 (2000) [C1]
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2000 |
Roberts-Thomson EL, Saunders H, Palmer SM, Powis DA, Dunkley PR, Bunn SJ, 'Ca2+ influx stimulated phospholipase C activity in bovine adrenal chromaffin cells: responses to K+ depolarization and histamine', European Journal of Pharmacology, 398 199-207 (2000) [C1]
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2000 |
Bobrovskaya L, Cheah TB, Dunkley PR, 'Angiotensin II-stimulated tyrosine hydroxylase activity and phosphorylation in bovine adrenal chromaffin cells', JOURNAL OF NEUROCHEMISTRY, 74 S38-S38 (2000) |
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2000 |
Lengyel I, Fieuw-Makaroff S, Hall AL, Sim AT, Rostas JA, Dunkley PR, 'Modulation of the Phosphorylation and Activity of Calcium/Calmodulin-Dependent Protein Kinase II by Zinc', Journal of Neurochemistry, 75 No. 2 594-605 (2000) [C1]
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Nova |
2000 |
Goncalves C-A, Gottfried C, Dunkley PR, 'The Use of Permeabilized Cells to Assay Protein Phosphorylatioon and Catecholamine Release', Neurochemical Research, 25 No. 6 885-894 (2000) [C1]
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2000 |
Graham ME, Dickson PW, Dunkley PR, Von Nagy-Felsobuki EI, 'Determination of Phosphorylation Levels of Tyrosine Hydroxylase by Electrospray Mass Spectrometry', Analytical Biochemistry, 280 1-7 (2000) [C1]
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1999 |
Bobrovskaya L, Cheah TB, Dunkley PR, 'Phorbol ester-mediated phosphorylation of tyrosine hydroxylase in bovine adrenal chromaffin cells', JOURNAL OF NEUROCHEMISTRY, 72 S25-S25 (1999) |
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1999 |
Lengyel I, Hall AL, Fieuw-Makaroff S, Sim ATR, Rostas JAP, Dunkley PR, 'Characterization of the zinc-dependent autophosphorylation sites on calcium/calmodulin-dependent protein kinase II', JOURNAL OF NEUROCHEMISTRY, 73 S33-S33 (1999)
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1999 |
Bobrovskaya L, Dickson P, Cheah TB, Dunkley PR, 'The role of mitogen-activated protein kinase in tyrosine hydroxylase phosphorylation.', JOURNAL OF NEUROCHEMISTRY, 73 S34-S34 (1999) |
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1999 |
Roberts-Thomson EL, Saunders HI, Dunkley PR, Bunn SJ, 'The effect of insulin on phospholipase C activity in bovine adrenal medullary chromaffin cells.', JOURNAL OF NEUROCHEMISTRY, 73 S50-S50 (1999) |
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1999 |
Cheah TB, Bobrovskaya L, Goncalves C-A, Hall RM, Elliot R, Lengyel I, et al., 'Simultaneous measurement of tyrosine hydroxylase activity and phosphorylation in bovine adrenal chomaffin cells', Journal of Neuroscience Methods, 87 167-174 (1999) [C1]
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1998 |
Bobrovskaya L, Cheah TB, Bunn SJ, Dunkley PR, 'Angiotensin II-mediated phosphorylation of tyrosine hydroxylase in bovine adrenal chromaffin cells', JOURNAL OF NEUROCHEMISTRY, 71 S60-S60 (1998) |
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1998 |
Bobrovskaya L, Cheah TB, Bunn SJ, Dunkley PR, 'Tyrosine hydroxylase in bovine adrenal chromaffin cells: Angiotensin II-stimulated activity and phosphorylation of Ser19, Ser31, and Ser40', Journal of Neurochemistry, 70 2565-2573 (1998) [C1]
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1998 |
Graham ME, Dickson PW, Dunkley PR, Von Nagy-Felsobuki EI, 'Characterisation of the phosphorylation of rat tyrosine hydroxylase using electrospray mass spectrometry', Rapid Communications in Mass Spectrometry, 12 746-748 (1998) [C1]
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1998 |
Graham ME, Dickson P, Dunkley P, Von Nagy-Felsobuki EI, 'Characterization of the Phosphorylation of Rat Tyrosine Hydroxylase using Electrospray Mass Spectrometry', Rapid Communications in Mass Spectrometry, 12 746-748 (1998) [C1]
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1997 |
Brent PJ, Herd L, Saunders H, Sim ATR, Dunkley PR, 'Protein phosphorylation and calcium uptake into rat forebrain synaptosomes: Modulation by the sigma (F) ligand 1,3-ditolylguanidine.', J.Neurochem, 68 2201-2211 (1997) [C1]
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1997 |
Goncalves CA, Hall A, Sim ATR, Bunn SJ, Marley P, Cheah TB, Dunkley PR, 'Tyrosine hydroxylase phosphorylation in digitonin permeabilised bovine adrenal chromaffin cells: The effect of protein kinase and phosphatase inhibitors on Ser-19 and Ser-40 phosphorylation.', J.Neurochem, 69 2387-2396 (1997) [C1]
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1997 |
Brent PJ, Herd L, Saunders H, Sim ATR, Dunkley PR, 'Protein phosphorylation and calcium uptake into rat forebrain synaptosomes: Modulation by the sigma (sigma) ligand, 1,3-ditolylguanidine', JOURNAL OF NEUROCHEMISTRY, 69 S206-S206 (1997) |
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1997 |
Goncalves CA, Hall A, Sim ATR, Bunn SJ, Marley PD, Cheah TB, Dunkley PR, 'Tyrosine hydroxylase phosphorylation in digitonin-permeabilised bovine adrenal chromaffin cells', JOURNAL OF NEUROCHEMISTRY, 69 S207-S207 (1997) |
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1997 |
Warden RA, Noltorp RS, Francis JL, Dunkley PR, OLoughlin EV, 'Vitamin A deficiency exacerbates methotrexate-induced jejunal injury in rats', JOURNAL OF NUTRITION, 127 770-776 (1997)
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1997 |
Bunn SJ, Dunkley PR, 'Histamine-stimulated phospholipase C signalling in the adrenal chromaffin cell: Effects on inositol phospholipid metabolism and tyrosine hydroxylase phosphorylation', CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 24 624-631 (1997)
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1996 |
Dunkley PR, Cote A, Harrison SM, Herd L, Hall A, Powis DA, 'Tyrosine hydroxylase phosphorylation in bovine adrenal chromaffin cells - Clonidine stimulates basal but inhibits nicotinic receptor evoked phosphorylation', BIOCHEMICAL PHARMACOLOGY, 51 239-245 (1996)
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1996 |
Warden RA, Strazzari MJ, Dunkley PR, OLoughlin EV, 'Vitamin A-deficient rats have only mild changes in jejunal structure and function', JOURNAL OF NUTRITION, 126 1817-1826 (1996)
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1996 |
Powis DA, OBrien KJ, Harrison SM, Jarvie PE, Dunkley PR, 'Mn2+ can substitute for Ca2+ in causing catecholamine secretion but not for increasing tyrosine hydroxylase phosphorylation in bovine adrenal chromaffin cells', CELL CALCIUM, 19 419-429 (1996)
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1996 |
Powis DA, O'Brien KJ, Harrison SM, Jarvie PE, Dunkley PR, 'Mn
The ability of the divalent cation manganese (Mn2+) to substitute for calcium (Ca2+) both in triggering catecholamine release and in stimulating catecholamine synthesis, as indica... [more]
The ability of the divalent cation manganese (Mn2+) to substitute for calcium (Ca2+) both in triggering catecholamine release and in stimulating catecholamine synthesis, as indicated by an increase in tyrosine hydroxylase (TOH) phosphorylation, has been determined in bovine adrenal medullary chromaffin cells maintained in tissue culture. Mn2+ was found to enter chromaffin cells through pathways activated by nicotinic receptor stimulation and potassium depolarisation, and via the Nai:Cao exchange mechanism in Na+-loaded cells. Like Ca2+, entry of Mn2+ through these pathways triggered immediate catecholamine release and, like Ca2+, maintained quantitatively comparable release at least up to 40 min. Unlike Ca2+, Mn2+ did not stimulate an increase in TOH phosphorylation in intact chromaffin cells, even over a prolonged time course, but Mn2+ did stimulate increased TOH phosphorylation in lysed cell preparations showing that its lack of effect in the intact cells was not due to inhibition of the specific phosphorylation pathway. In lysed cell preparations, Mn2+ stimulated also phosphorylation of a different spectrum of proteins to Ca2+, and of the same proteins to different extents. In particular, P80 (MARCKS protein) was more intensely phosphorylated in the presence of Mn2+ than in the presence of Ca2+. Since TOH phosphorylation always occurs when intracellular Ca2+ is increased, the absence of an increase with Mn2+ indicates that none of its intracellular effects could have occurred as a consequence of Mn2+ mobilisation of intracellular Ca2+. In summary, the data show that Mn2+ is a surrogate for Ca2+ in triggering and maintaining catecholamine release, but does not substitute for Ca2+ in stimulating TOH phosphorylation.
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1996 |
Brent PJ, Saunders H, Dunkley PR, 'Intrasynaptosomal free calcium levels in rat forebrain synaptosomes: Modulation by sigma (sigma) receptor ligands', NEUROSCIENCE LETTERS, 211 138-142 (1996)
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1996 |
Lengyel I, Nichol KA, Sim ATR, Bennett MR, Dunkley PR, Rostas JAP, 'Characterization of protein kinase and phosphatase systems in chick ciliary ganglion', NEUROSCIENCE, 70 577-588 (1996)
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1996 |
Amini SA, Dunkley PR, Murdoch RN, 'Teratogenic effects of ethanol in the Quackenbush Special mouse', DRUG AND ALCOHOL DEPENDENCE, 41 61-69 (1996)
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1996 |
Amini SA, Dunstan RH, Dunkley PR, Murdoch RN, 'Oxidative stress and the fetotoxicity of alcohol consumption during pregnancy', FREE RADICAL BIOLOGY AND MEDICINE, 21 357-365 (1996)
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1995 |
BRENT PJ, HAYNES H, JARVIE PE, MUDGE L, SIM ATR, DUNKLEY PR, 'PHOSPHORYLATION OF SYNAPSIN I AND DYNAMIN IN RAT FOREBRAIN SYNAPTOSOMES - MODULATION BY SIGMA (SIGMA) LIGANDS', NEUROSCIENCE LETTERS, 191 71-74 (1995)
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1995 |
SITGES M, DUNKLEY PR, CHIN LM, 'A ROLE FOR CALCIUM/CALMODULIN KINASE(S) IN THE REGULATION OF GABA EXOCYTOSIS', NEUROCHEMICAL RESEARCH, 20 245-252 (1995)
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1995 |
Jarvie PE, Dunkley PR, 'Characterization of calcium/calmodulin-stimulated protein kinase II.', Methods in molecular biology (Clifton, N.J.), 41 239-259 (1995)
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1995 |
Amini SA, Walsh K, Dunstan R, Dunkley PR, Murdoch RN, 'Maternal hepatic, endometrial, and embryonic levels of Zn, Mg, Cu, and Fe following alcohol consumption during pregnancy in QS mice', RESEARCH COMMUNICATIONS IN ALCOHOL AND SUBSTANCES OF ABUSE, 16 207-219 (1995)
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1995 |
WARDEN R, FRANCIS L, DUNKLEY P, OLOUGHLIN E, 'VITAMIN-A-DEFICIENCY POTENTIATES METHOTREXATE (MTX) ENTEROPATHY IN RATS', GASTROENTEROLOGY, 108 A762-A762 (1995) |
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1995 |
BUNN SJ, SIM ATR, HERD LM, AUSTIN LM, DUNKLEY PR, 'TYROSINE-HYDROXYLASE PHOSPHORYLATION IN BOVINE ADRENAL CHROMAFFIN CELLS - THE ROLE OF INTRACELLULAR CA2+ IN THE HISTAMINE H-1 RECEPTOR-STIMULATED PHOSPHORYLATION OF SER(8), SER(19), SER(31), AND SER(40)', JOURNAL OF NEUROCHEMISTRY, 64 1370-1378 (1995)
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1995 |
DUNKLEY PR, BUNN SJ, SIM ATR, AUSTIN L, HERD L, HALL A, BUCKENHAM AJ, 'INTRACELLULAR CALCIUM-DEPENDENT PHOSPHORYLATION OF TYROSINE-HYDROXYLASE IN BOVINE ADRENAL CHROMAFFIN CELLS', JOURNAL OF NEUROCHEMISTRY, 65 S5-S5 (1995) |
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1995 |
BRENT PJ, MUDGE L, DUNKLEY PR, 'MODULATION OF PROTEIN-PHOSPHORYLATION BY THE SELECTIVE SIGMA-LIGAND DTG IN RAT CORTICAL SYNAPTOSOMES IS CALCIUM-DEPENDENT AND INHIBITED BY THE SIGMA-ANTAGONIST RIMCAZOLE', JOURNAL OF NEUROCHEMISTRY, 65 S14-S14 (1995)
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1995 |
BUCKENHAM AJ, GONCALVES CA, HALL A, DUNKLEY PR, 'CALCIUM/CALMODULIN-DEPENDENT PROTEIN-KINASE-II IN INTACT BOVINE ADRENAL CHROMAFFIN CELLS', JOURNAL OF NEUROCHEMISTRY, 65 S16-S16 (1995) |
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1995 |
SITGES M, DUNKLEY PR, CHIU LM, 'A ROLE FOR CALCIUM-CALMODULIN KINASE(S) IN THE REGULATION OF GABA EXOCYTOSIS', JOURNAL OF NEUROCHEMISTRY, 65 S19-S19 (1995) |
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1995 |
BUNN SJ, SAUNDERS HI, DUNKLEY PR, 'HISTAMINE-STIMULATED INOSITOL PHOSPHOLIPID-METABOLISM IN BOVINE ADRENAL-MEDULLARY CELLS - A KINETIC-ANALYSIS', JOURNAL OF NEUROCHEMISTRY, 65 626-635 (1995)
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1994 |
Dunkley PR, 'Advances in Second Messenger and Phosphoprotein Research, Vol. 27 : Model Systems in Signal Transduction edited by S. Shenolikar and A. C. Nairn. Raven Press, New York, 1993, ISBN 0-88167-975-5, 224 pp., $95.00', Journal of Neurochemistry, 62 2062-2062 (1994)
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1994 |
WARDEN RA, STRAZZARI MJ, DUNKLEY PR, OLOUGHLIN EV, 'THE EFFECT OF VITAMIN-A-DEFICIENCY ON INTESTINAL STRUCTURE AND FUNCTION', GASTROENTEROLOGY, 106 A639-A639 (1994) |
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1993 |
BUNN SJ, BOYD TE, DUNKLEY PR, 'KINETIC-ANALYSIS OF HISTAMINE-STIMULATED INOSITOL PHOSPHOLIPID-METABOLISM IN BOVINE ADRENAL CHROMAFFIN CELLS', JOURNAL OF NEUROCHEMISTRY, 61 S104-S104 (1993) |
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1993 |
SIM ATR, RATCLIFFE E, DUNKLEY PR, ROSTAS JAP, 'MEMBRANE-BOUND FORMS OF PROTEIN PHOSPHATASE TYPE-1 AND TYPE-2A HAVE LOWER ACTIVITY THAN CYTOSOLIC FORMS IN BRAIN', JOURNAL OF NEUROCHEMISTRY, 61 S136-S136 (1993)
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1993 |
BUNN SJ, AUSTIN LM, HERD LM, DUNKLEY PR, 'HISTAMINERGIC PHOSPHORYLATION OF TYROSINE-HYDROXYLASE IN BOVINE ADRENAL CHROMAFFIN CELLS', JOURNAL OF NEUROCHEMISTRY, 61 S153-S153 (1993) |
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1993 |
LENGYEL I, NICHOL KA, BENNETT MR, ROSTAS JAP, DUNKLEY PR, 'PROTEIN-PHOSPHORYLATION IN CHICK CILIARY GANGLION', JOURNAL OF NEUROCHEMISTRY, 61 S221-S221 (1993)
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1993 |
SIM ATR, LLOYD HGE, JARVIE PE, MORRISON M, ROSTAS JAP, DUNKLEY PR, 'SYNAPTOSOMAL AMINO-ACID RELEASE - EFFECT OF INHIBITING PROTEIN PHOSPHATASES WITH OKADAIC ACID', NEUROSCIENCE LETTERS, 160 181-184 (1993)
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1992 |
KIDD GJ, HEATH JW, TRAPP BD, DUNKLEY PR, 'MYELIN SHEATH SURVIVAL AFTER GUANETHIDINE-INDUCED AXONAL DEGENERATION', JOURNAL OF CELL BIOLOGY, 116 395-403 (1992)
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1992 |
BUNN SJ, HARRISON SM, DUNKLEY PR, 'PROTEIN-PHOSPHORYLATION IN BOVINE ADRENAL-MEDULLARY CHROMAFFIN CELLS - HISTAMINE-STIMULATED PHOSPHORYLATION OF TYROSINE-HYDROXYLASE', JOURNAL OF NEUROCHEMISTRY, 59 164-174 (1992)
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1992 |
ROSTAS JAP, DUNKLEY PR, 'MULTIPLE FORMS AND DISTRIBUTION OF CALCIUM/CALMODULIN-STIMULATED PROTEIN KINASE-II IN BRAIN', JOURNAL OF NEUROCHEMISTRY, 59 1191-1202 (1992)
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1992 |
PRESEK P, JESSEN S, DREYER F, JARVIE PE, FINDIK D, DUNKLEY PR, 'TETANUS TOXIN INHIBITS DEPOLARIZATION-STIMULATED PROTEIN-PHOSPHORYLATION IN RAT CORTICAL SYNAPTOSOMES - EFFECT ON SYNAPSIN-I PHOSPHORYLATION AND TRANSLOCATION', JOURNAL OF NEUROCHEMISTRY, 59 1336-1343 (1992)
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1992 |
CAMPBELL EM, REDMAN S, DUNKLEY PR, MOFFITT PS, 'THE USE OF PORTABLE BLOOD-GLUCOSE MONITORS BY TRAINED LAY OPERATORS', MEDICAL JOURNAL OF AUSTRALIA, 157 446-448 (1992)
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1991 |
BUNN SJ, DUNKLEY PR, 'OPIOID INHIBITION OF NICOTINE-INDUCED CA-45(2+)-UPTAKE INTO CULTURED BOVINE ADRENAL-MEDULLARY CELLS', BIOCHEMICAL PHARMACOLOGY, 41 715-722 (1991)
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1991 |
Thorne B, Wonnacott S, Dunkley PR, 'Isolation of Hippocampal Synaptosomes on Percoll Gradients: Cholinergic Markers and Ligand Binding Sites', Journal of Neurochemistry, 56 479-484 (1991)
Abstract: The S1 Percoll procedure, devised empirically for cortical tissue, provides highly purified, functionally viable synaptosomes on a four-step Percoll gradient. Here, for ... [more]
Abstract: The S1 Percoll procedure, devised empirically for cortical tissue, provides highly purified, functionally viable synaptosomes on a four-step Percoll gradient. Here, for the first time, the procedure has been applied to rat hippocampus, and the gradient fractions have been analysed with respect to cholinergic markers and the synaptosomal index, lactate dehydrogenase. The presynaptic cholinergic markers choline acetyltransferase and [3H]choline uptake were most enriched in fraction 4. In contrast, acetylcholinesterase activity was broadly distributed across the gradient, consistent with the separation of synaptic plasma membranes (in fractions 1 and 2) from synaptosomes (in fractions 3 and 4). This is supported by the recovery of muscarinic binding sites labelled with [3H]quinuclidinylbenzilate in fractions 1 and 2. (-)-[3H]-Nicotine binding sites, however, were most enriched in fraction 4, consistent with their predominantly presynaptic localisation in the CNS. These results demonstrate the applicability of the S1 Percoll method to discrete brain regions for the recovery of homogeneous and viable synaptosome fractions. The separation of presynaptic terminals from post-synaptic membranes is a further advantage of this technique. Copyright © 1991, Wiley Blackwell. All rights reserved
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1991 |
KIDD GJ, HEATH JW, TRAPP BD, LITTLE GJ, DUNKLEY PR, 'MYELIN SHEATH MAINTENANCE IN THE ABSENCE OF AXONS', ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 633 549-549 (1991) |
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1991 |
SIM ATR, DUNKLEY PR, JARVIE PE, ROSTAS JAP, 'MODULATION OF SYNAPTOSOMAL PROTEIN-PHOSPHORYLATION DEPHOSPHORYLATION BY CALCIUM IS ANTAGONIZED BY INHIBITION OF PROTEIN PHOSPHATASES WITH OKADAIC ACID', NEUROSCIENCE LETTERS, 126 203-206 (1991)
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1991 |
HEATH JW, KIDD GJ, TRAPP BD, DUNKLEY PR, 'MYELIN MAINTENANCE BY SCHWANN-CELLS IN THE ABSENCE OF AXONS', NEUROSCIENCE LETTERS, 128 277-280 (1991)
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1991 |
JEITNER TM, JARVIE PE, COSTA M, ROSTAS JAP, DUNKLEY PR, 'PROTEIN-PHOSPHORYLATION IN GUINEA-PIG MYENTERIC GANGLIA AND BRAIN - PRESENCE OF CALMODULIN KINASE-II, PROTEIN-KINASE-C AND CYCLIC-AMP KINASE AND CHARACTERIZATION OF MAJOR PHOSPHOPROTEINS', NEUROSCIENCE, 40 555-569 (1991)
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1991 |
DUNKLEY PR, 'AUTOPHOSPHORYLATION OF NEURONAL CALCIUM CALMODULIN-STIMULATED PROTEIN KINASE-II', MOLECULAR NEUROBIOLOGY, 5 179-202 (1991)
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1991 |
DUNKLEY PR, COTE A, HARRISON SM, 'AUTOPHOSPHORYLATION OF CALMODULIN-STIMULATED PROTEIN KINASE-II IN INTACT SYNAPTOSOMES', JOURNAL OF MOLECULAR NEUROSCIENCE, 2 193-201 (1991)
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1989 |
O'Dea K, Dunkley P, MacMahon B, Seal J, Birkbeck J, Court J, et al., 'Methods and models for introducing nutrition into the medical curriculum', Medical Journal of Australia, 151 (1989)
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1989 |
ROSTAS JAP, BRENT VA, SECCOMBE M, WEINBERGER RP, DUNKLEY PR, 'PURIFICATION AND CHARACTERIZATION OF CALMODULIN-STIMULATED PROTEIN KINASE-II FROM 2-DAY AND ADULT CHICKEN FOREBRAIN', JOURNAL OF MOLECULAR NEUROSCIENCE, 1 93-104 (1989)
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1989 |
Rostas JAP, Brent VA, Seccombe M, Weinberger RP, Dunkley PR, 'Purification and characterization of calmodulin-stimulated protein kinase II from two-day and adult chicken forebrain', Journal of Molecular Neuroscience, 1 93-104 (1989)
Soluble calmodulin-stimulated protein kinase II has been purified from 2-day and adult chicken forebrain. At both ages the holoenzyme eluted from a Superose-6B column with an appa... [more]
Soluble calmodulin-stimulated protein kinase II has been purified from 2-day and adult chicken forebrain. At both ages the holoenzyme eluted from a Superose-6B column with an apparent molecular weight of approximately 700,000 daltons and contained three subunits. The subunits were found to be the counterparts of the alpha, beta, and beta' subunits of the enzyme purified from adult rat brain in that they had one-dimensional phosphopeptide maps that were indistinguishable from those of the corresponding subunit in the rat enzyme and they migrated in SDS-polyacrylamide gels with the same apparent molecular weights. However, the doublet formed by the beta subunit was much more clearly resolved in the chicken enzyme and the beta' subunit, which was much more abundant in the adult chicken than in the adult rat, was also found to be a doublet. The ratio of the concentrations of the alpha and beta subunits changed during development. By autoradiography following autophosphorylation, the alpha:beta ratios of the 2-day and adult enzymes were 0.89 ± 0.07 and 1.92 ± 0.26, respectively; by silver staining the alpha:beta ratios were 0.95 ± 0.11 and 1.85 ± 0.17, respectively. The concentration of the beta' subunit was equal to that of the beta subunit at both ages. Autophosphorylation produced a decrease in the electrophoretic mobility of the alpha and beta subunits in SDS-polyacrylamide gels and a marked decrease in the calcium dependence of the substrate phosphorylation activity of the enzyme at both ages. The purified enzyme from chicken brain appeared to be more stable under standard in vitro assay conditions than the rat enzyme, and this was particularly so for the enzyme from 2-day forebrain. © 1989 Humana Press Inc.
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1989 |
Court J, Wahlqvist M, Truswell S, Daniels L, Helman T, MacMahon B, et al., 'Objectives for nutrition in the medical curriculum', Medical Journal of Australia, 151 (1989)
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1988 |
DUNKLEY PR, HEATH JW, HARRISON SM, JARVIE PE, GLENFIELD PJ, ROSTAS JAP, 'A RAPID PERCOLL GRADIENT PROCEDURE FOR ISOLATION OF SYNAPTOSOMES DIRECTLY FROM AN S-1 FRACTION - HOMOGENEITY AND MORPHOLOGY OF SUBCELLULAR-FRACTIONS', BRAIN RESEARCH, 441 59-71 (1988)
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1988 |
HARRISON SM, JARVIE PE, DUNKLEY PR, 'A RAPID PERCOLL GRADIENT PROCEDURE FOR ISOLATION OF SYNAPTOSOMES DIRECTLY FROM AN S-1 FRACTION - VIABILITY OF SUBCELLULAR-FRACTIONS', BRAIN RESEARCH, 441 72-80 (1988)
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1988 |
DUNKLEY PR, JARVIE PE, ROSTAS JAP, 'DISTRIBUTION OF CALMODULIN-STIMULATED AND CYCLIC AMP-STIMULATED PROTEIN-KINASES IN SYNAPTOSOMES', JOURNAL OF NEUROCHEMISTRY, 51 57-68 (1988)
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1988 |
THORNE B, IRONS J, LUNT GG, WONNACOTT S, DUNKLEY PR, 'COMPARISON OF METHODS FOR RAPID ISOLATION OF SYNAPTOSOMES FROM BRAIN-REGIONS, FOR UPTAKE AND RELEASE STUDIES', BIOCHEMICAL SOCIETY TRANSACTIONS, 16 309-310 (1988)
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1987 |
ROBINSON PJ, DUNKLEY PR, 'ALTERED PROTEIN-PHOSPHORYLATION IN INTACT RAT CORTICAL SYNAPTOSOMES AFTER INVIVO ADMINISTRATION OF FLUPHENAZINE', BIOCHEMICAL PHARMACOLOGY, 36 2203-2208 (1987)
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1987 |
ROBINSON PJ, HAUPTSCHEIN R, LOVENBERG W, DUNKLEY PR, 'DEPHOSPHORYLATION OF SYNAPTOSOMAL PROTEINS P96 AND P139 IS REGULATED BY BOTH DEPOLARIZATION AND CALCIUM, BUT NOT BY A RISE IN CYTOSOLIC CALCIUM ALONE', JOURNAL OF NEUROCHEMISTRY, 48 187-195 (1987)
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1987 |
Rostas JAP, Brent V, Dunkley PR, 'The effect of calmodulin and autophosphorylation on the activity of calmodulin-stimulated protein kinase II', Neuroscience Research Communications, 1 3-8 (1987)
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1986 |
DUNKLEY PR, JARVIE PE, HEATH JW, KIDD GJ, ROSTAS JAP, 'A RAPID METHOD FOR ISOLATION OF SYNAPTOSOMES ON PERCOLL GRADIENTS', BRAIN RESEARCH, 372 115-129 (1986)
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1986 |
KIDD GJ, HEATH JW, DUNKLEY PR, 'SELECTIVE DEGENERATION OF MYELINATED SYMPATHETIC-NERVES FOLLOWING TREATMENT WITH GUANETHIDINE', JOURNAL OF ANATOMY, 149 250-250 (1986) |
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1986 |
DUNKLEY PR, BAKER CM, ROBINSON PJ, 'DEPOLARIZATION-DEPENDENT PROTEIN-PHOSPHORYLATION IN RAT CORTICAL SYNAPTOSOMES - CHARACTERIZATION OF ACTIVE PROTEIN-KINASES BY PHOSPHOPEPTIDE ANALYSIS OF SUBSTRATES', JOURNAL OF NEUROCHEMISTRY, 46 1692-1703 (1986)
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1986 |
DUNKLEY PR, ROBINSON PJ, 'DEPOLARIZATION-DEPENDENT PROTEIN-PHOSPHORYLATION IN SYNAPTOSOMES - MECHANISMS AND SIGNIFICANCE', PROGRESS IN BRAIN RESEARCH, 69 273-293 (1986)
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1986 |
ROSTAS JAP, WEINBERGER RP, DUNKLEY PR, 'MULTIPLE POOLS AND MULTIPLE FORMS OF CALMODULIN-STIMULATED PROTEIN-KINASE DURING DEVELOPMENT - RELATIONSHIP TO POSTSYNAPTIC DENSITIES', PROGRESS IN BRAIN RESEARCH, 69 355-371 (1986)
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1986 |
KIDD GJ, HEATH JW, DUNKLEY PR, 'DEGENERATION OF MYELINATED SYMPATHETIC-NERVE FIBERS FOLLOWING TREATMENT WITH GUANETHIDINE', JOURNAL OF NEUROCYTOLOGY, 15 561-572 (1986)
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1986 |
ROSTAS JAP, BRENT VA, HEATH JW, NEAME RLB, POWIS DA, WEINBERGER RP, DUNKLEY PR, 'THE SUBCELLULAR-DISTRIBUTION OF A MEMBRANE-BOUND CALMODULIN-STIMULATED PROTEIN-KINASE', NEUROCHEMICAL RESEARCH, 11 253-268 (1986)
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1985 |
ROBINSON PJ, DUNKLEY PR, 'DEPOLARIZATION-DEPENDENT PROTEIN-PHOSPHORYLATION AND DEPHOSPHORYLATION IN RAT CORTICAL SYNAPTOSOMES IS MODULATED BY CALCIUM', JOURNAL OF NEUROCHEMISTRY, 44 338-348 (1985)
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1984 |
ROBINSON PJ, JARVIE PE, DUNKLEY PR, 'DEPOLARIZATION-DEPENDENT PROTEIN-PHOSPHORYLATION IN RAT CORTICAL SYNAPTOSOMES IS INHIBITED BY FLUPHENAZINE AT A STEP AFTER CALCIUM ENTRY', JOURNAL OF NEUROCHEMISTRY, 43 659-667 (1984)
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1984 |
NELL JA, DUNKLEY PR, 'EFFECTS OF TEMPERATURE, NUTRITIONAL FACTORS AND SALINITY ON THE UPTAKE OF L-METHIONINE BY THE SYDNEY ROCK OYSTER SACCOSTREA-COMMERCIALIS', MARINE BIOLOGY, 80 335-339 (1984)
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1984 |
ROBINSON PJ, DUNKLEY PR, 'IS THERE A ROLE FOR CALCIUM-STIMULATED PROTEIN-PHOSPHORYLATION IN NEUROTRANSMITTER RELEASE', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 16 S40-S40 (1984) |
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1984 |
WEINBERGER RP, DUNKLEY PR, ROSTAS JAP, 'DEVELOPMENTAL ACCUMULATION OF A CALMODULIN STIMULATED PROTEIN-KINASE IN SYNAPTIC-MEMBRANES', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 16 87-87 (1984)
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1983 |
NELL JA, SKEEL ME, DUNKLEY P, 'UPTAKE OF SOME DISSOLVED ORGANIC NUTRIENTS BY THE SYDNEY ROCK OYSTER SACCOSTREA-COMMERCIALIS', MARINE BIOLOGY, 74 313-318 (1983)
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1983 |
ROSTAS JAP, BRENT VA, DUNKLEY PR, 'THE MAJOR CALMODULIN-STIMULATED PHOSPHOPROTEIN OF SYNAPTIC JUNCTIONS AND THE MAJOR POST-SYNAPTIC DENSITY PROTEIN ARE DISTINCT', NEUROSCIENCE LETTERS, 43 161-165 (1983)
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1983 |
ROBINSON PJ, DUNKLEY PR, 'DEPOLARIZATION-DEPENDENT PROTEIN-PHOSPHORYLATION IN RAT CORTICAL SYNAPTOSOMES - FACTORS DETERMINING THE MAGNITUDE OF THE RESPONSE', JOURNAL OF NEUROCHEMISTRY, 41 909-918 (1983)
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1983 |
ROBINSON PJ, DUNKLEY PR, 'DEPOLARIZATION-DEPENDENT PROTEIN-PHOSPHORYLATION IN RAT CORTICAL SYNAPTOSOMES - THE EFFECTS OF CALCIUM, STRONTIUM AND BARIUM', NEUROSCIENCE LETTERS, 43 85-90 (1983)
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1983 |
DUNKLEY PR, ROBINSON PJ, 'THE INVITRO PHOSPHORYLATION OF ACTIN FROM RAT CEREBRAL-CORTEX', NEUROCHEMICAL RESEARCH, 8 865-871 (1983)
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1981 |
DUNKLEY PR, ROBINSON PJ, 'PREINCUBATION OF SUB-CELLULAR FRACTIONS FROM RAT CEREBRAL-CORTEX INACTIVATES PROTEIN-PHOSPHORYLATION', BIOCHEMICAL JOURNAL, 199 269-272 (1981)
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1981 |
DUNKLEY PR, ROBINSON PJ, 'CALCIUM-STIMULATED PROTEIN-KINASES FROM RAT CEREBRAL-CORTEX ARE INACTIVATED BY PRE-INCUBATION', BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 102 1196-1202 (1981)
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1980 |
DUNKLEY PR, 'IS PROTEIN-PHOSPHORYLATION INVOLVED IN NEUROTRANSMISSION IN THE CENTRAL NERVOUS-SYSTEM', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 13 Q5-Q5 (1980) |
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1980 |
DUNKLEY PR, 'PHOSPHORYLATION OF SYNAPTOSOMAL PROTEINS INVITRO - THE EFFECT OF HYPOTONIC LYSIS AND TRITON X-100', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 13 118-118 (1980) |
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1979 |
Dunkley PR, Anderson JM, 'Isolation of the light-harvesting chlorophyll a b-protein complex from thylakoid membranes of barley by adsorption chromatography on controlled-pore glass', Archives of Biochemistry and Biophysics, 193 469-477 (1979)
The light-harvesting chlorophyll a b-protein complex has been isolated from barley thylakoids by a rapid, single-step procedure involving adsorption chromatography on controlled-p... [more]
The light-harvesting chlorophyll a b-protein complex has been isolated from barley thylakoids by a rapid, single-step procedure involving adsorption chromatography on controlled-pore glass columns. The Triton X-100-solubilized complex contains a polypeptide of apparent molecular weight, 26,000; the 0.25% Triton X-100 light-harvesting chlorophyll a b-protein has spectral characteristics consistent with its assumed in vivo state. On the same column free chlorophyll and carotenoids have been separated from chlorophyll-protein complex 1, but this complex contained many polypeptides other than those associated with chlorophyll. This method is potentially suitable for the isolation of other thylakoid membrane proteins. It may also be generally applicable for fractionation of intrinsic membrane proteins from other sources and for separation of mixed Triton X-100-lipid micelles. © 1979.
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1979 |
Dunkley PR, Anderson JM, 'The light-harvesting chlorophyll a b-protein complex from barley thylakoid membranes. Polypeptide composition and characterization of an oligomer', BBA - Bioenergetics, 545 175-187 (1979)
Electrophoretic analysis by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis showed that the light-harvesting chlorophyll a b-protein complex of barley thylakoids ... [more]
Electrophoretic analysis by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis showed that the light-harvesting chlorophyll a b-protein complex of barley thylakoids contains only one polypeptide of apparent molecular weight 26 000. The barley mutant, deficient in chlorophyll b and this light-harvesting complex, lacks this polypeptide. The addition of a nonionic detergent, Triton X-100, to the sodium dodecyl solubilization buffer prior to SDS polyacrylamide tube gel electrophoresis, allowed separation of a relatively stable complex, characterized as an oligomeric form of the light-harvesting complex. The oligomer also contained a polypeptide with an apparent molecular weight of 26 000. The absorption and fluorescence spectral properties of the oligomer are similar to those of the monomer. It is suggested that the oligomer of the light-harvesting chlorophyll a b-protein is closer to the in vivo form rather than the monomer. © 1979.
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1977 |
Dunkley PR, Holmes H, Rodnight R, 'Phosphorylation of synaptic membrane proteins from ox cerebral cortex in vitro. Preparation of fractions enriched in phosphorylated proteins by using extraction with detergents and urea, and gel filtration', Biochemical Journal, 163 369-378 (1977)
Synaptic-membrane fragments from ox cerebral cortex contain basal and cyclic AMP-stimulated protein kinase(s) that transfer 32P from [¿-32P]ATP to hydroxyl groups of serine and th... [more]
Synaptic-membrane fragments from ox cerebral cortex contain basal and cyclic AMP-stimulated protein kinase(s) that transfer 32P from [¿-32P]ATP to hydroxyl groups of serine and threonine residues in membrane-protein substrates. In the present work, labelled membrane fragments were partitioned into soluble fractions with Triton X-100, Nonidet P. 40, sodium deoxycholate and urea, and the distribution of 32P-labelled protein in the fractions was determined by polyacrylamide-gel electrophoresis and radioautography. A high percentage of phosphorylated protein substrates remained insoluble, including those whose phosphorylation was most highly stimulated by cyclic AMP. Whole membrane fragments and samples prepared by detergent extraction were fractionated on Sepharose 6B columns in the presence of low concentrations of sodium dodecyl sulphate and pooled fractions were analysed by polyacrylamide-gel electrophoresis and radioautography. Phosphorylated proteins were fractionated on the basis of their molecular weight, but homogeneous protein was not obtained.
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1976 |
Dunkley PR, Holmes H, Rodnight R, 'Phosphorylation of synaptic membrane proteins from ox cerebral cortex in vitro. Partition of substrates and protein kinase activities with Triton X 100', Biochemical Journal, 157 661-666 (1976)
Synaptic membrane fragments from ox cerebral cortex contain basal and cyclic AMP stimulated protein kinase activity catalysing the phosphorylation of endogenous substrates. Extrac... [more]
Synaptic membrane fragments from ox cerebral cortex contain basal and cyclic AMP stimulated protein kinase activity catalysing the phosphorylation of endogenous substrates. Extraction of membrane fragments with Triton X 100 solubilized less than 20% of the kinase activity and left the major part of the endogenous substrates in the insoluble fraction.
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1974 |
Carnegie PR, Dunkley PR, Kemp BE, Murray AW, 'Phosphorylation of selected serine and threonine residues in myelin basic protein by endogenous and exogenous protein kinases', Nature, 249 147-150 (1974)
THERE is considerable interest in phosphoprotein metabolism in the brain and the effects of neurotransmitters on the turnover of phosphate in membrane proteins1. Emphasis has been... [more]
THERE is considerable interest in phosphoprotein metabolism in the brain and the effects of neurotransmitters on the turnover of phosphate in membrane proteins1. Emphasis has been placed on the phosphorylation of proteins of synaptosomal membranes, but in addition myelin comprises a considerable proportion of the total substrate activity for a soluble protein kinase of brain2. In the following paper Miyamoto et al.3 show that this kinase, which is stimulated by adenosine 3', 5'-cycle monophosphate (cyclic AMP), phosphorylates the basic protein of myelin. Besides being a substrate for this soluble kinase the basic protein was found to be phosphorylated by an endogenous kinase of myelin3. Carnegie et al.4 demonstrated that a protein kinase from rabbit muscle would selectively phosphorylate certain serine and threonine residues in the basic proteins of rat and human myelin. Soluble protein kinases from brain and muscle seem to phosphorylate similar sites in histones5. We present evidence here that the endogenous protein kinase of myelin phosphorylates the basic protein, but not the proteolipid protein, and that the site of phosphorylation by this endogenous kinase is quite different from the site phosphorylated by soluble protein kinase. © 1974 Nature Publishing Group.
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1974 |
Dunkley PR, Carnegie PR, 'Amino acid sequence of the smaller basic protein from rat brain myelin', Biochemical Journal, 141 243-255 (1974)
The complete amino acid sequence of the smaller basic protein from rat brain myelin was determined. This protein differs from myelin basic proteins of other species in having a de... [more]
The complete amino acid sequence of the smaller basic protein from rat brain myelin was determined. This protein differs from myelin basic proteins of other species in having a deletion of a polypeptide of 40 amino acid residues from the center of the molecule. A detailed comparison is made of the constant and variable regions in a group of myelin basic proteins from 6 species. An arginine residue in the rat protein was found to be partially methylated. The ratio of methylated to unmethylated arginine at this position differed from that found for the human basic protein. 3 tryptic peptides were isolated in more than 1 form. The differences between the 2 forms of each peptide are discussed in relation to the electrophoretic heterogeneity of myelin basic proteins, which is known to occur at alkaline pH values.
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1974 |
Lennon VA, Dunkley PR, 'Humoral and cell-mediated immune responses of Lewis rats to syngeneic basic protein of myelin', International Archives of Allergy and Immunology, 47 598-608 (1974)
In Lewis rats, injection of syngeneic basic protein of myelin with Freund¿s complete adjuvant and pertussis vaccine induced clinical and histological signs of experimental autoimm... [more]
In Lewis rats, injection of syngeneic basic protein of myelin with Freund¿s complete adjuvant and pertussis vaccine induced clinical and histological signs of experimental autoimmune encephalomyelitis (EAE), humoral antibody, and cell-mediated immunity to the basic protein. Antigenic cross reactivity between the encephalitogenic basic proteins of rat and human was demonstrated. Injection into Lewis rats of graded doses of rat or human basic protein induced dose-related levels of serum antibody and severity of EAE. In growing rats, low doses of rat basic protein (0.1¿1.0 µg) did not induce clinical or histological signs of EAE, nor detectable serum antibody, but did induce loss of weight. In rats receiving larger doses of basic protein, weight loss was marked, and the mean levels of serum antibody rose abruptly when the rats began to regain weight. A possible function in vivo of serum antibody to myelin basic protein may be to reestablish immunological tolerance to the basic protein at the level of the immunocompetent cell; the amount of antibody required to restore tolerance may depend on the dose of immunogen used to initiate the autoimmune response. © 1974 by S. Karger AG, Basel.
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1973 |
Dunkley PR, Coates AS, Carnegie PR, 'Communications. Encephalitogenic activity of peptides from the smaller basic protein of rat myelin.', Journal of Immunology, 110 1699-1701 (1973)
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1973 |
Carnegie PR, Kemp BE, Dunkley PR, Murray AW, 'Phosphorylation of myelin basic protein by an adenosine 3':5' cyclic monophosphate dependent protein kinase', Biochemical Journal, 135 569-572 (1973)
Myelin basic protein was shown to be a substrate for protein kinase from rabbit muscle. One of the major sites of phosphorylation was the serine residue in the sequence Gly Arg Gl... [more]
Myelin basic protein was shown to be a substrate for protein kinase from rabbit muscle. One of the major sites of phosphorylation was the serine residue in the sequence Gly Arg Gly Leu Ser Leu. The arginine residue in this sequence is known to be a substrate for a protein methylase.
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